| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 201 | Holdemania filiformis | ACGACAACGCGACACTGT | CGGTTCACGGTTGCCTTTG | 59.90 | 60.01 | 281 | 79 |
100.00%
|
50.00%
|
| Primer 202 | Holdemania filiformis | TCAACAACGCCTTGCTTGC | TTTGCGGACAAGCCAGGA | 59.93 | 59.49 | 183 | 78 |
100.00%
|
50.00%
|
| Primer 203 | Holdemania filiformis | TCAGGCTCAGGTTGAAGCC | TGGACAGCTTGCCGTTGA | 59.62 | 59.49 | 200 | 78 |
100.00%
|
50.00%
|
| Primer 204 | Holdemania filiformis | GCGGTCTTTGTTGCGGATC | TGAACGTCCGCTGCTTGA | 59.87 | 59.58 | 184 | 78 |
100.00%
|
50.00%
|
| Primer 205 | Holdemania filiformis | GCGGTCTTTGTTGCGGAT | TGAACGTCCGCTGCTTGA | 58.74 | 59.58 | 184 | 78 |
100.00%
|
50.00%
|
| Primer 206 | Holdemania filiformis | TGGTGCTGATCAATCCGCA | TGAACGTCCGCTGCTTGA | 59.70 | 59.58 | 159 | 78 |
100.00%
|
50.00%
|
| Primer 207 | Holdemania filiformis | AGCGCTGAAGGCAATGGT | TCCTGAATCAGTGCCAGCG | 59.97 | 60.08 | 183 | 78 |
100.00%
|
50.00%
|
| Primer 208 | Holdemania filiformis | GCGTAATCCGGATGGTCGA | TTCCGGTTGGCCATGCAT | 59.64 | 59.96 | 152 | 78 |
100.00%
|
50.00%
|
| Primer 209 | Holdemania filiformis | ATTTCCTGCAGGCCAGCT | TTGCCCAGTTCACGGATCG | 59.23 | 60.38 | 157 | 78 |
100.00%
|
50.00%
|
| Primer 210 | Holdemania filiformis | GAAGTCGCAACGGTTCTGC | GCCTAACTTGGTGTGCGGA | 59.80 | 60.30 | 255 | 78 |
100.00%
|
50.00%
|